Biography:

Youhoon Chong has completed his PhD at the age of 33 years from University of Georgia and postdoctoral studies from The Scripps Research Institute. He is the chairman of the department of integrative bioscience & biotechnology, Konkuk University. He has published more than 140 papers in reputed journals.    

Abstract:

(-)-Epigallocatechin-3-gallate (EGCG) is known as a mitochondria-targeted molecule that can prevent mitochondrial deterioration and induce mitochondrial biogenesis by modulating key regulators of mitochondrial metabolism. In this study, we tackled whether derivatization of EGCG could result in enhancement of its effects on mitochondrial biogenesis. EGCG, EGCG peracetate (AcEGCG), and its 4″-O-alkyl substituted congeners prepared by previously reported procedures were biologically evaluated. Interestingly, EGCG and AcEGCG were only marginally effective in inducing mitochondrial biogenesis, while AcEGCG congeners with an alkyl group at the 4″-O position showed significantly increased biological activity compared to their parent compound. Among these series, compound 3f with a methyl-branched carbonate chain at the 4″-O position of the AcEGCG scaffold showed the most enhancement in inducing mitochondrial biogenesis. Hepa1-6 cells treated with 3f exhibited increases in both mitochondrial mass (1.5 times) and relative mtDNA content to nDNA (1.5 times). As a mitochondrial biogenesis enhancer, 3f also increased expression levels of regulators for mitochondrial function, including PGC-1α (4.0 fold), p-AMPK (2.5 fold), SIRT1 (4.2 fold), ERRα (1.8 fold), NRF-1 (1.6 fold), NRF-2 (1.7 fold), and mtTFA (2.0 folds). Investigation of oxidative phosphorylation by mitochondria in the presence of 3f revealed that 3f increased NAD⁺/NADH ratio, the amount of cytochrome c, ATP synthesis, and oxygen consumption in Hepa1-6 cells by 2.2, 1.4, 1.5, and 2.1 folds, respectively. Taken together, these results warrant extensive structure-activity relationship study for EGCG derivatives to develop novel mitochondrial biogenesis enhancers.

Biography:

Flavia Laffleur, is an assistant prfoessorand a senior researcher of Drug Delivery in the Department of Pharmacy at LFU Innsbruck, Austria. Flavia Laffleur published over 68 publications and gave oral presentations on several international conferences. From 2010 until 2013 she completed her doctoral thesis focused on smart drug delivery systems. Since 2013, she is a senior researcher at the Department of Pharmaceutical Technology in Innsbruck. Since 2017, Dr. Flavia Laffleur is a researcher at the MIT, in Boston, Massachusetts. She received several awards, including LesmüllerStiftung award and the Galenus Foundation Technology Award. Currently Dr. Laffleur´s research focusses on mucosal drug delivery as well as smart delivery systems to overcome biological barriers 

Abstract:

Predominantly, the majority of fungal infections (dermal and nail) are caused by dermatophytes, such as Trichophyton rubrum known as one of the most prominent . Among fungal infections, nail infections or onychomycosis exhibit the most difficulties and limitations in their treatment. Onychomycosis affects around 5-10% of the population in the world. Onychomycosis is a common infection of the nail caused by dermatophyte affecting mostly toenails in adults being associated with limited treatment options. In this study novel dosage forms were prepared and evaluated for their suitability in treatment of onychomycosis. Films were prepared comprising polymeric excipients such as chitosan, (hydroxypropyl)methyl cellulose, hydroxyethyl-cellulose, carboxymethylcellulose according to solvent evaporation method. Developed formulations were evaluated in terms of physical appearance, stability and adhesiveness. Furthermore skin and nail irritation studies were conducted. Five potential formulations (F1-F5) were designed while F1 and F4 exhibited the most promising results in terms of stability with 26 min and 40.67 min, respectively, and suitability in nail application. F1 as the most favorable dosage form revealed with 2.9438 kg/m/s in terms of adhesive force the most adhesive properties in contrast to the other preparations. All formulations were found to be non-skin irritating and safe to use. Taken together, these findings suggest novel designed films containing polymeric excipients as a fruitful platform for the treatment in onychomycosis. 

Biography:

HF is the director of Department of Plastic and Reconstructive Surgery, Hamamatsu University School of Medicine. He published some articles on drug delivery in the following journals.

1. Development of three-microneedle device for hypodermic drug delivery and clinical application.      Plast Reconstr Surg, 2012.

2. Application of a three-microneedle device for the delivery of local anesthetics. Patient Preference and Adherence, 2015.

Abstract:

Background: We have developed a new device with multiple fine needles fabricated with a bevel angle to release a drug broadly and homogeneously into the skin in a horizontal fashion and reported in the article. It led us to develop another type of multiple needles to release a drug in the epidermis or upper dermis.  The intradermal (ID) route for vaccination represents an effective alternative to subcutaneous (SC)/intramuscular administration to induce protective immunity. However, a critical issue associated with ID vaccination is the precise delivery of solution in the upper dermis, which ensures enhanced immunity.

Methods: We fabricated a hollow microneedle unit made of poly-glycolic acid by injection molding and bonding, and developed a dedicated prototype injector. To ensure ID delivery of solution, the injected site was macroscopically and microscopically examined. Serum immunoglobulin G antibody production was measured by enzyme immunoassay and compared in groups of rats following either ID delivery with microneedles or SC administration with a 27-G stainless needle of graded vaccine doses.

Results: The unit used a tandem array of six microneedles, each with a side delivery hole, and a conduit inside for solution. Microneedles installed in the injector punctured the skin with the aid of a spring. Injection of solution formed a wheal due to ID distribution. Histologically, a wedge-shaped skin defect in the upper skin corresponded to each puncture site. Antibody titers following vaccinations on days 1 and 8 were significantly higher with ID injection than with SC delivery on day 15 and every 7 days thereafter until day 36 with mumps vaccination, and until day 36 with varicella vaccination.

Conclusions: The microneedle unit presented here delivered solution intradermally without any difficulty and evoked antibody responses against viruses even with the reduced vaccine volume. Our findings confirm promising results of ID delivery as an immunogenic option to enhance vaccination efficacy.

Biography:

Yukihiro Akao has completed his PhD, United Graduate School of Drug Discovery and Medical Information Sciences

Abstract:

Despite considerable research on K-Ras inhibitors, none had been developed until now. We synthesized nuclease-resistant synthetic miR-143 (miR-143#12), which strongly silenced K-Ras, its effector signal molecules AKT and ERKs, and the K-Ras activator Sos1. We examined the anti-proliferative effect of miR-143#12 and the mechanism in human colon cancer DLD-1 cell (G13D) and other cell types harboring K-Ras mutations. Cell growth was markedly suppressed in a concentration-dependent manner by miR-143#12 (IC₅₀: 1.32 nM) with a decreased in the K-Ras mRNA level. Interestingly, this mRNA level was also down-regulated by either a PI3K/AKT or MEK inhibitor, which finding indicates a positive circuit of K-Ras mRNA expression. MiR-143#12 silenced cytoplasmic K-Ras mRNA expression and impaired the positive circuit by directly targeting AKT and ERK mRNAs. Combination treatment with miR-143#12 and a low-dose EGFR inhibitor induced a synergistic inhibition of growth with a marked inactivation of both PI3K/AKT and MAPK/ERK signaling pathways. However, silencing K-Ras by siR-KRas instead of miR-143#12 did not induce this synergism by the combined treatment with the EGFR inhibitor. Thus, miR-143#12 perturbed the K-Ras expression system and K-Ras activation by silencing SOS1 and resultantly, recovered the efficacy of the EGFR inhibitors. In vivo results also supported those of the in vitro experiments. The extremely potent miR-143#12 enabled us to well understand K-Ras networks and shut them down by combination treatment with this miRNA and EGFR inhibitor in K-Ras-driven colon cancer cells

Biography:

Hiba Natsheh is currently a PhD candidate under the supervision of Prof. Elka Touitou at the Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem. Prof. Touitou (elka.touitou@mail.huji.ac.il) is well recognized for her novel approaches for enhanced transmucosal drug delivery.

Abstract:

The nasal route for drug administration can be considered a safe, convenient and noninvasive alternative to the conventional oral and parenteral routes. However, by using classic carriers, the delivery to brain of hydrophilic molecules, peptides and proteins is poor. We have designed and investigated a new nasal carrier for enhanced drug delivery to brain, we call Phospholipid Magnesome. The system contains soft phospholipid vesicles, composed of phospholipid, water, propylene glycol and magnesium salt.  In addition, the carrier contains the mucoadhesive polymer, alginate. Electron microscopy, calorimetry and dynamic light scattering measurements show that the system is composed of soft multilamellar nanosized vesicles. The ability of the carrier's vesicles to entrap both lipophilic and hydrophilic molecules is evidenced by CLSM and ultracentrifugation studies. The mucoadhesivity test results of the Phospholipid Magnesome carrier following in vitro application on porcine nasal mucosa, indicate a prolonged contact time of the drug with the nasal membrane as compared to control. Effective delivery of various molecules to brain, Rohdamine 6G (R6G), Insulin and Epidermal Growth Factor (EGF) was demonstrated by two methods, Multiphoton Microscopy and Near Infrared (NIR) imaging. Moreover, results of a pharmacodynamic study measuring the antinociceptive effect of Oxytocin administrated nasally to an animal model indicate the efficiency of the Phospholipid Magnesome as compared to three control compositions. In an additional study in animals, nasal administration of Insulin, resulted in a strong and prolonged hypoglycemic effect for the drug incorporated in the new carrier but not for control systems. Histopathological analysis of nasal mucosa following sub-chronic administration indicates the local safety of the system.

In conclusion, the results of this investigation suggest that Phospholipid Magnesome nasal carrier is able to improve drug effect, probably by a combined mechanism, absorption enhancement and prolongation of mucosal contact

Biography:

Abstract:

Objective: The most important objective of this presentation is to attract attention that sometimes the prescription of drugs become abusive and the phenomenon is really very difficult to be controlled. 

Material and methods: The idea of this presentation appeared after many clinical observations from my medical practice, regarding a new phenomenon. The intention of the patients is to consult many doctors, from different specialties in a short period of time, for diverse health issues and because every physician prescribe his therapeutic scheme, in the end the patient accumulate many therapeutic schemes in same time and in conclusion a lot of drugs, many unnecessary. Interactions between these accumulations of drugs from different classes are indeed very difficult to monitor and quantify. The truth is that many unnecessary drugs appear in complexes therapeutic scheme, most of them difficult to can be stopped sudden. Often the patients came with severe allergic reactions, which after investigations remain of unknown etiology and in reality the interactions between different classes of medications represent the real cause of allergy.

Results and discussions: Because the patients consults many physicians in a short period of time, abusive therapeutics schemes appear in the medical practice and the phenomenon is really difficult to be controlled. More than that, sometimes the patient administers from his own initiative other drugs and don’t recognize and complicate more the situation.

Conclusion: The most important conclusion of this presentation is that the side effects between different classes of drugs represent a reality and the abusive therapeutic schemes with many unnecessary drugs must to be stopped.

Biography:

Hazal Ezgi Gültekin graduated from Gazi University Faculty of Pharmacy, at the age of 23. She is doing her PhD in the Department of Pharmaceutical Technology of Gazi University since 2014. Her area of interests are 3D printing, fused deposition modeling and hot melt extrusion. She has studies on the development of dosage forms by FDM 3DP technology. She participated various scientific conferences and meetings with her studies.

Abstract:

3D printing (3DP) is a layer-by-layer production process which is used to manufacture 3D objects utilizing computer aided design data. In pharmaceutical area, fabrication of personalized dosage forms is one of the important advantages of this technology. Fused deposition modeling (FDM) is a widely used 3DP technology. In FDM, an extruded polymer filament passes through a heated nozzle and after moltening it deposits onto a build plate to create a hard object. Pramipexole is a commonly used drug for the symptomatic treatment of Parkinson’s disease.The aim of this study was to prepare pramipexole loaded 3DP tablets.

    Firstly, drug loaded polymer filaments were prepared by hot melt extrusion (Filabot Filament Extruder). After that, by using these filaments 3DP tablets were printed via 3D printer (MakerBot^® Replicator_ 2X Experimental 3D Printer). In the present study, scanning electron microscope (SEM) images of the filaments and the 3DP tablets were taken, the physico-pharmaceutical properties (weight uniformity, dimensions and friability) of the 3DP tablets were evaluated and in vitro dissolution profiles of commercial tablets and 3DP tablets were compared.

    In conclusion, drug loaded 3DP tablets were successfully fabricated by FDM. This study demonstrates that fused deposition modeling 3D printing is an effective technology for the development of personalized dosage forms on the treatment of diseases.

Biography:

Gamze Rüzgar Özemre graduated from Ankara University in 2012. She is studying for her doctoral studies at Gazi University School of Pharmacy Department of Pharmaceutical Technology. Her areas of interest are nanotechnology and drug delivery systems. She has studies and poster presentations on these topics

Abstract:

Heparin and it’s derivates, as an anti-coagulant, are considered the drug of choice in  the treatment of deep vein thrombosis (DVT) and pulmonary embolism. Due to high molecular weight and high negative charge density, oral bioavailability of heparin is limited and insufficient to provide the desired clinical therapeutic effects.

The electrospinning technique, a straightforward procedure applied in the production of nanomaterials, requires a basic experimental setup composed by a high voltage source which provides high electrical field between the dip of a needle and a grounded target at few centimeters from ejection of charged jet.Electrospun nanofibers show great promise for developing many types of novel drug delivery systems (DDS) due to their special characteristics and the simple but useful and effective top-down fabricating process.

The aim of this study is the development and evaluation of efficacy of nanofibers to increase the oral bioavailability of low molecular weight heparin. Core-shell nanofibers were prepared using the co-axial electrospinning (NE- 300 Laboratory Scale Electrospinning Unit, Inovenso LTD, Turkey). Therefore low molecular weight heparin which is in the core of nanofibers were protected from degradation and absorbed through the intestine by using different cationic polymers which are insoluble in acidic pH in the shell of nanofibers.

Biography:

Emel Mataracı Kara has completed her PhD at the age of 30 years from Istanbul University and postdoctoral studies from Istanbul University Faculty of Pharmacy. She has published more than 15 papers in reputed journals.

Abstract:

Bacterial biofilms cause chronic infections because they show increased tolerance to antibiotics and disinfectant chemicals as well as host's immune defense mechanisms. Because of the rising in multidrug resistance from infectious agents, there is a prompted interest for the development of new antimicrobial agents and new therapeutic strategies to combat the infections caused by the resistant bacterial biofilm infections.

Antimicrobial cationic peptides (AMPs) have attracted attention as alternative antibiotics due to their prospective potency, rapid action, and borad sprectrum of activities against Gram negative and positive bacteria viruses, fungi and parasites. AMPs can be found as major component of the innate immun systems of most living organisms, including insects, plants, microorganisms, and mammals, to protect against environmental microorganisms. In addition, they exhibit multiple mechanisms of action and, consequently, a low potential to induce the resistance, which allows the limited use of other antibiotics.

Therefore we investigated the in vitro pharmacokinetic activities of antimicrobial cationic peptides (indolicidin and nisin) alone or in combination with antibiotics (daptomycin, linezolid, teicoplanin, ciprofloxacin) against MRSA biofilms. With checkerboard technique, synergistic interactions against MRSA biofilms were frequent with almost all antibiotic-AMP combinations. The time kill curve studies demonstrated that synergistic interaction occured most frequently when using nisin+daptomycin/ciprofloxacin, indolicidin+teicoplanin. No antagonism was observed.

Consequently, use of a combination of antimicrobial agents can provide a synergistic effect and may help prevent or delay the emergence of resistance. AMPs seem to be good candidates for further investigations in the treatment of MRSA biofilms, alone or in combination with antibiotics.

Biography:

He is the professor at Al-Azhar University

Abstract:

Bifidobacterium represent one of the major genera of the intestinal tract of human and animals used as probiotics in dairy and nondairy foods for restore the intestinal microflora which confers a health benefit. The identification of Bifidobacterium by phenotypic features is commonly unreliable, time, money, and effort consuming. We sought to improve the Bifidobacterium identification method based on molecular level to identify probiotic bacteria in complex microbial communities. The application of 16S-23S rRNA oligonucleotide primers is the best and most reliable, rapid, and precise species and sub species identification approach. The ribosomal intergenic spacer region (ISR) located between the highly conserved 16S rRNA and 23S rRNA shows a high degree of variation in length and sequence and potential for intra species discrimination and providing the phylogenetic Relationship of the Genus Bifidobacterium spp. Results showed that one of the two primer sets Bflac2-Bflac5 species specific gives positive results differentiating between B. animalis ssp. Lactis isolated from breast fed infants milk of human and that isolated from feces of breast fed infant and detecting reference strain for B. animalis ssp. Lactis DSM10140. DNA sequences of the two strains were submitted to the Genbank NCBI under accession number (KT758845) named as B. animalis ssp. Lactis Egm1 (Egyptian milk) and accession number (KT758846) named as Egf1 Egyptian feces while the second primer give false positive result. Also, we aim to obtain patent protection under Intellectual property rights (IPRs) for B. animalis ssp.Lactis which was isolated from Egyptian resources to be used for a better and healthier food and dairy products

Biography:

Dr Nicolaes has completed his PhD at the age of 28 years from the Maastricht University and postdoctoral studies from Lund University (Sweden). He is a unit leader in the department of Biochemistry at the Cardiovascular Research Institute Maastricht, has an appointment at the Academic Medical Centre Amsterdam and is CSO for Matisse Pharmaceuticals BV, a start-up university spin-off. He has published more than 80 peer-reviewed papers in the fields of coagulation, atherosclerosis, inflammation and drug design.

Abstract:

Chronic inflammation of the arterial wall, as occurs in atherosclerosis, may lead to stroke or myocardial infarction. One of the interactions that are known to drive this inflammatory reaction in atherosclerosis is that between CD40 protein and its ligand CD40L. For CD40 to elicit intracellular signalling, it needs to recruit adaptor proteins called tumour necrosis factor receptor-associated factors (TRAFs). Interactions of CD40 with TRAF6 but not those with  TRAF2/3/5 are critically involved in atherosclerosis progression and plaque stabilization; which makes theCD40-TRAF6 interaction a downstream target for potential inhibition of inflammatory processes in atherosclerosis.

We have used a hierarchical protocol of structure-based virtual ligand screening (SBVLS) to discover small drug-like inhibitors of the CD40-TRAF6 interaction and combined in silico researches with in vitro cell-based assays and biophysical methods. Top hit molecules were administered to Apoe-/- mice models of initial and of established atherosclerosis. In both models hit molecules, named TRAF-STOPs reduced total plaque area and changed the plaque phenotype to a more stable phenotype. Intravital microscopy showed a reduction in leukocyte recruitment. We furthermore used rHDL nanoparticles loaded with one of the compounds to specifically target TRAF STOPs to macrophages, resulting in clear attenuation of atherosclerosis.

The compounds inhibited CD40-TRAF6 interaction by direct binding to TRAF6 C-domain and reduced progression of atherosclerosis by inhibition of chemokine-mediated leukocyte influx into the arterial wall, while the molecules did not impair classical immune pathways of CD40. Our results indicate possibilities of long-term therapeutic inhibition of CD40-TRAF6 interactions in atherosclerosis and possibly other inflammatory diseases.

Biography:

Gleb N. Zyuz`kov is a Scientist Secretary of Institute, Head of the laboratory of pathology and experimental therapy  (Goldberg Research Institute of Pharmacology and Regenerative Medicine), Tomsk National Research Medical Center. Ph.D (2003),  Doctor of Medicine (2006), Professor of Russian Academy of Sciences (2016). Author of 56 patents. International Award of Elsevier`s "SciVal/Scopus Award Russia» (2012). Dr. Zyuz′kov G.N. is: auditor of the Tomsk branch of «National society for regenerative medicine» (Russia), a member of «Russian scientific society of Pharmacology» and «Tomsk Professors Society».

Abstract:

Advances in the field of cellular technologies have led to the possibility of developing a new direction of targeted therapy in regenerative medicine - "Strategy of Pharmacological Regulation of Intracellular Signal Transduction in Regenerator-competent Cells". The role of NF-кB, IKK, PKC, PKB, PI3K, ERK ½, p38, adenylate cyclase, PKA, JAKs, STAT3, JNK, p53 in the realization of functioning progenitor elements of different classes and cells of tissue microenvironment was studied in vitro by means of cultural, immunological and other methods. On the models of posthypoxic encephalopathy, skin wound and cytostatic myelosuppression in experimental animals the therapeutic effects and mechanisms of action of modifiers of signal molecules activity were studied. The specificity of the involvement of a number of signaling molecules in the regulation of cell cycle and development of progenitor cells of various classes, as well as in the production of humoral factors by microenvironment cells was revealed. The neuroregenerative effects of JNK inhibitors associated with activation of neural stem cells of brain were shown on the model of encephalopathy. An algorithm and approaches for estimating the potential efficiency and many-sided selectivity of the modifiers of signaling molecules activity as targeted hemostimulators were developed. The effectiveness of various targeted pharmacological agents determined by the selective effect on different types of regenerative-competent cells was demonstrated on the models of cytostatic myelosuppression of various genesis. The perspective of using intracellular signaling molecules in regenerative-competent cells as targets of drugs for regenerative medicine was shown..

Biography:

Radostina Alexandrova Graduated with honors in Biochemistry and Microbiology, Sofia University “St. Kl. Ohridski” (SU); MSc and PhD in Virology, lecturer in SU and PhD School of BAS; > 160  publications, > 550 abstracts, 3 Book chapters;  Postdoctoral training in Slovakia, Hungary, Denmark; Leading researcher in 5 national and 10 bilateral projects; MC Member of  five COST Actions; Member of Union of Scientists in Bulgaria, Bulgarian Society of Anatomy, Secretary-Treasurer of the Immunological Society; Member of the Organizing/Scientific Committees of 45 scientific forums; Editor/Member of editorial boards of 2 national and 4 international journals; Editor of the proceeding books of two Workshops.

Abstract:

The aim of our study was to evaluate the cytotoxic activity of the following compounds: six complexes of Zn(II)/Au(I) and Zn(II)/Ag(I) with Salen, Salampy and Salampy and  eight complexes of Cu(II) and Co(II) with Schiff bases derived by a condensation reaction of  Ð¾-Vanillin with S-Tyrosine, L-Threonine, DL-Tryptophan or L-Serine.

            As model systems were used six permanent cell lines: MCF-7 and MDA-MB-231 (human breast cancer); HeLa (human cervical carcinoma); LACC-SF-Mc29 (chicken hepatoma); LSR-SF-SR (rat sarcoma) and Lep-3 (non-tumor human embryonic fibroblastoid cells).

The investigations were performed by MTT test, neutral red uptake cytotoxicity assay, crystal violet staining, double staining with propidium iodide and acridine orange, Comet assay, Annexin V – FITC test (in short-term experiments, 3-72h, with monolayer cultures) and colony-forming method (in long-term experiments, 30-40 days, with 3D cell colonies). The  compounds were examined in ROS generating systems, DPPH test was also performed.

Our results indicate that the complexes investigated decrease viability and proliferation of the treated cells in a time- and concentration-dependent manner. The most pronounced cytotoxic agents are Zn(II)/Au(I) complexes with Salen, Salampy and Saldmen (active in the concentration range 0.05 - 5 µg/ml), followed by Zn(II)/Ag(I) complexes of the same ligands (active in the concentration range 1-20 µg/ml). The CC₅₀ of Zn(II)/Au(I) and Zn(II)/Ag(I) complexes are lower than those of cisplatin. Cu(II) complexes are effective applied at concentrations of 10-200 µg/ml. Co(II) complexes show the lowest rate of cytotoxicity (active in a concentration range 50 - 400 µg/ml).   Cu(II) and Co(II)   complexes do not express antioxidant activity.

Biography:

Khedr Naglaa has completed his PhD at the age of 25 years from Andhra University and postdoctoral studies from Stanford University School of Medicine. He is the director of a premier Bio-Soft service organization. He has published more than 25 papers in reputed journals and has been serving as an editorial board member of repute. (Up to 100 words)     
 

Abstract:

Acute pancreatitis (AP) is a common inflammatory disease mediated by damage in acinar cells and subsequent pancreatic inflammation with infiltration of leukocytes. The pancreatic renin-angiotensin system may play an important role in the pathogenesis of AP. The present study aimed to investigate the possible role of captopril (CAP), an angiotensin-converting enzyme inhibitor, in attenuating L-arginine-induced AP in a rat model and to elucidate the underlying molecular mechanisms. Forty-eight adult male Wister rats were divided into 4 equal groups: control group (rats received vehicle orally for 10 days), AP group (3 g/kg L-arginine, single i.p.) on 10^th day of the experiment, CAP group (50 mg/kg captopril, orally, once daily) and MP group (30 mg/kg methylprednisolone, orally, once daily). CAP and MP were administered for 10 days prior to L-arginine injection. Then rats were sacrificed 24 h after L-arginine injection. Inflammatory biomarkers; pancreatic tumor necrosis factor-alpha (TNF-α) concentration, myeloperoxidase (MPO) activity and inducible nitric oxide synthase (iNOS) gene expression were determined. Oxidative stress biomarkers; nitric oxide (NO) and reduced glutathione (GSH) concentrations were assayed. In addition, serum α-amylase and lipase activities were measured and histopathological studies of the pancreas were done. CAP treatment significantly reduced TNF-α, MPO activity, NO and downregulated iNOS gene expression compared to AP group. CAP treatment significantly increaseed pancreatic GSH and ameliorated the histological changes of AP. Captopril treatment may have a protective role in AP rat model which is comparable to MP treatment.

Biography:

Rabindra Nath Das he is a professor at  The University of Burdwan, India.

Abstract:

Objectives: The report presents the effects of dobutamine dose on the cardiac parameters such as blood pressures (basal, systolic, diastolic & maximum),  heart rates (basal, peak & maximum), baseline cardiac ejection fraction, ejection fraction on dobutamine dose.  

Background: There is a little literature about the effects of dobutamine dose on the cardiac parameters.

Materials and Methods: The effects of dobutamine dose on the  cardiac parameters have been examined based on a real echocardiography stress data set, collected at University of California, Los Angeles on 558 patients with 31 explanatory variables/ factors. The distribution of the considered cardiac parameters is gamma with non-constant variance. So, they have been analyzed by joint generalized linear gamma  models.  

Results: The mean basal blood pressure (BBP) decreases as the double product of  maximum heart rate (MHR) & maximum blood pressure (MBP) at dobutamine dose (DPMAXDO) (P<0.001) increases, while the variance of BBP increases as the DPMAXDO (P<0.001) increases. The mean systolic blood pressure (SBP) increases as the dobutamine dose (DOSE) (P=0.032) increases, while the mean SBP increases as the DPMAXDO (P<0.001) decreases. The mean MBP increases as the DPMAXDO (P<0.001) increases. The mean baseline cardiac ejection fraction (BEF) decreases as the DOSE (P=0.025) increases. The mean ejection fraction on dobutamine dose (DOBEF) increases as the DOSE (P=0.011) increases, while the variance of DOBEF increases as the dobutamine dose at maximum double product (DOBDOSE) (P=0.001) decreases. The mean basal heart rate (BHR) increases as the DPMAXDO (P<0.001), or DOBDOSE (P=0.074) decreases. The mean peak heart rate (PHR), or maximum heart rate (MHR) increases as the DPMAXDO (P<0.001) increases, while the variance of PHR, (MHR) increases as the DOBDOSE (P<0.001) decreases (increases). On the other hand, dobutamine dose is associated with many cardiac parameters such as SBP, MBP, new myocardial infraction (new MI), history of MI (hxofMI)etc.              

Conclusions: Only the dobutamine dose effects are observed on SBP, MBP, DOBEF, newMI, histMI, etc, while the joint effects of dobutamine (DPMAXDO and DOBDOSE) are observed on each cardiac parameters. The results are new inputs in the dobutamine dose study literature.

Biography:

Ahmed Awd is currently working as professor in Department of Chemical Engineering in Egypt.

Abstract:

Lactobacillus represent one of the major genera of the intestinal tract of human and animals and are used, as probiotics, in dairy and non-dairy foods to restore the intestinal microflora which confer a health benefit. After an adaptation period for 7 days, the first group was fed on basal diet (80 g- for each rat group /day) and served as control I, while the second group was offered basal diet plus standardized buffalo's milk (40 ml. for each rat group / day) and served as control II. The other groups were fed on 80 grams of basal diet for each rat group / day and 40 ml. / day for each rat group, buffalo's milk plus one of the following Lactobacillus strains respectively L.casei strain AZ1, L.rhamnosus strain AZ1 and L.gasseri strain AZ1. Furthermore, supplementation of diets with fermented milk products cultured with L. casei KY123805 or L. rhamnosus KY123789 resulted in noticeable decreases in Total cholesterol, HDL- cholesterol and triglycerides levels at the end of the experiment (28 days) as compared to dry diet (control I). Species of lactobacilli occurring in intestinal tract deconjugate both taurocholic and glycocholic acids, such serum cholesterol levels when it is considered that deconjugated bile acids function more poorly in supporting adsorption of lipids from the intestinal tract than deconjugated ones, this could result in reduce adsorption of cholesterol from the intestines and thus influence its serum level. Therefore, the main target of the present investigation was to isolate and identify some local isolates belonging to genera Lactobacillus. Also, the isolated strains have been screened in order to define their characteristics that would be as probiotic strains or not. Furthermore, the long-term goal of this work is to registering patent protection for some Lactobacillus spp. isolated from local Egyptian resources to increase the additive values of the Egyptian microbial wealth and well use it in the industrial healthy dairy products and pharmaceutical.  

Biography:

Abstract:

The purpose of this review is to summarize the pertinent literature published in the present era regarding ulcerogenic effectors, and all available therapeutic concepts in this regard including; different physiological/pathological changes in response to H. pylori infection, nonsteroidal anti-inflammatory drugs (NSAID), bile acids, nitric oxide, copper complexes, acid pump inhibitors, histamine blockers, curcuminoids, cytokines and/or growth factors and finally probiotics. Because of the partial understanding of gastric ulcer pathogenesis three major hypotheses were strongly speculated and widely documented. Firstly, the hyperacidity hypothesis entailing the disturbance of the gastric acid, histamine, gastrin and somatostatin. Secondly, the eicosanoid imbalance hypothesis exploiting changes in the microcirculation through the vasoconstrictor eicosanoids such as TXA2 and vasodilator cytoprotectant eicosanoids such as PGE2. Thirdly, the infective hypothesis implementing H. pylori as the major pathogenic effectror for the gastroduodenal ulceration. In fact, all of the previous effectors could be involved and possibly employing inflammatory/antiinflammatory, oxidative stress and/or angiogenic disturbance.