Scientific Program

Conference Series LLC Ltd invites all the participants across the globe to attend 19th Annual Congress on Pharmaceutics & Drug Delivery Systems Geneva, Switzerland.

Day 1 :

Keynote Forum

Yuxin Pei

Northwest A&F University, Yangling, Shaanxi 712100, China

Keynote: pH and H2O2 dual-responsive nanoparticles for monitoring intracellular protein Delivery

Time : 09:30-10:15

Euro Pharmaceutics 2020 International Conference Keynote Speaker Yuxin Pei photo
Biography:

Yuxin Pei did her PhD in 1998 in material science at China Textile University (Donghua University at present) in Shanghai. After a two-year postdoctoral training at Zhejiang University, she moved to Europe and worked as post-doctoral fellow at Lund University, Royal Institute of Technology, Technical University of Denmark, and Heidelberg University. Since 2010, she has been a full professor of chemistry at Northwest A&F University. Her current research interest is drug delivery systems and target.

 

Abstract:

Protein therapy holds enormous promise for cancer treatment due to its potential high potency. However, its clinical application is severely limited due to poor membrane permeability of proteins as well as lack of efficient delivery vehicles and methods of real-time protein-tracking. Here a pH and H2O2 dual-responsive NIRF (near-infrared fluorescence) turn-on protein delivery system incorporating an NIRF turn-on probe and protein into one single nanoparticle was rationally designed and fabricated based on tumor microenvironment for monitoring intracellular protein delivery. The nanoparticles can be taken up efficiently by A549 cells, where protein release and NIRF recovery happen simultaneously in response to low pH and excessive H2O2. More importantly, the delivery of protein via such a delivery system didn’t compromise on the activity of the protein. Thus, we provide a new approach to fabricate biocompatible and efficient stimuli-responsive turn-on systems for monitoring intracellular protein delivery. We believe that the strategy developed in this work may find broad applications in drug release monitoring and cancer cell imaging.

 

Protein therapy holds enormous promise for cancer treatment due to its potential high potency. However, its clinical application is severely limited due to poor membrane permeability of proteins as well as lack of efficient delivery vehicles and methods of real-time protein-tracking. Here a pH and H2O2 dual-responsive NIRF (near-infrared fluorescence) turn-on protein delivery system incorporating an NIRF turn-on probe and protein into one single nanoparticle was rationally designed and fabricated based on tumor microenvironment for monitoring intracellular protein delivery. The nanoparticles can be taken up efficiently by A549 cells, where protein release and NIRF recovery happen simultaneously in response to low pH and excessive H2O2. More importantly, the delivery of protein via such a delivery system didn’t compromise on the activity of the protein. Thus, we provide a new approach to fabricate biocompatible and efficient stimuli-responsive turn-on systems for monitoring intracellular protein delivery. We believe that the strategy developed in this work may find broad applications in drug release monitoring and cancer cell imaging.

 

 

Keynote Forum

Radostina Alexandrova

Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria

Keynote: Metal [Zn(II), Co(II), Ni(II)] complexes with kojic acid: do they help us to fight against cancer?

Time : 10:15-11:00

Euro Pharmaceutics 2020 International Conference Keynote Speaker Radostina Alexandrova photo
Biography:

Prof. Radostina Alexandrova has graduated with honors in Biochemistry and Microbiology, Sofia University “St. Kl. Ohridski” (SU) in 1991; MSc and PhD in Virology; Postdoctoral training in Slovakia, Hungary, Denmark, Iceland; lecturer in SU and PhD School of Bulgarian Academy of Sciences (BAS). She is a team-leader in the Department of Pathology, IEMPAM-BAS. She has published more than 150 papers in reputed journals and conference proceedings.

 

Abstract:

The aim of our study was to evaluate the influence of newly synthesized  complexes of Zn(II), Co(II) and Ni(II) with kojic acid on viability and proliferation of cultured human (HeLa cervical carcinoma), rat (sarcoma LSR-SF-SR) and avian (liver cancer LSCC-SF-Mc29) cancer cells. Short-term experiments (24-72 h, with monolayer cell cultures) by thiazolyl blue tetrazolium bromide (MTT) test, neutral red uptake assay (NR), crystal violet staining (CV), double staining with acridine orange and propidium iodide, AnnexinV/FITC method as well as long-term experiments (14 days, with 3D cancer cell colonies) by 3D-colony forming method were carried out to investigate the cytotoxic activity of the compounds. The results obtained revealed that the compounds examined decreased viability and inhibited 2D and 3D growth of the treated cells in a time- and concentration-dependent manner. Co(II) complex with kojic acid (CoKoj) was found to be the most promising cytotoxic agent in human HeLa and rat LSR-SF-SR cells whereas ZnKoj showed the highest effectivity in chicken LSCC-SF-Mc29 cells. NiKoj exhibited the lowest cytotoxicity. Cytopathological changes and apoptosis were observed in the cells cultivated in the presence of the compounds tested.